| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NPW was used as the immunogen for the NPW antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NPW Antibody / Neuropeptide W is a anti-NPW Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: NPW
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, NPW antibody identifies a 69-amino-acid precursor protein that is processed into two active peptides, NPW-23 and NPW-30, differing by their C-terminal truncation. These peptides are expressed in specific regions of the hypothalamus, brainstem, and peripheral tissues such as adrenal gland and stomach. NPW acts as an endogenous ligand for NPBWR1/2, coupling primarily to Galphai/o proteins and modulating cyclic AMP levels and calcium flux.
The NPW gene is located on chromosome 17p13.1 and encodes a prepropeptide that undergoes proteolytic processing and amidation to generate mature NPW peptides. In the central nervous system, NPW contributes to the regulation of feeding behavior, energy expenditure, and stress hormone release. It acts synergistically with leptin and insulin pathways to balance energy intake and expenditure. NPW also modulates arousal, locomotion, and nociceptive processing through brainstem circuits.
In peripheral tissues, NPW influences cardiovascular tone, adrenal steroidogenesis, and glucose metabolism. Its expression is regulated by nutritional state and stress exposure, reflecting its role in maintaining physiological equilibrium. In metabolic research, NPW is recognized as a peptide integrating hypothalamic signaling with peripheral energy regulation. Dysregulated NPW expression has been associated with obesity, stress-related disorders, and endocrine dysfunction.
NPW antibody is widely used in neuroendocrinology, metabolism, and behavioral neuroscience research. It is suitable for immunohistochemistry, ELISA, and western blotting to detect NPW peptide distribution in brain and peripheral tissues. This antibody supports studies of neuropeptide signaling, hypothalamic regulation, and energy balance. In translational research, NPW serves as a potential biomarker for metabolic and stress-related conditions.
Structurally, NPW peptides adopt flexible conformations stabilized by amidation and hydrophobic residues, allowing receptor binding with high affinity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
