| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NR1D1 was used as the immunogen for the NR1D1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NR1D1 Antibody / Nuclear receptor subfamily 1 group D member 1 is a anti-NR1D1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: NR1D1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
Functionally, NR1D1 antibody recognizes a 65 kDa nuclear protein that represses transcription by binding to Rev-erb response elements (ROREs) within target gene promoters. NR1D1 lacks the classical activation domain found in many nuclear receptors and instead recruits corepressor complexes such as NCoR and HDAC3 to silence transcription. Its oscillatory expression pattern forms part of the molecular circadian clock, antagonizing the activity of the transcriptional activator ROR alpha to maintain rhythmic gene expression. NR1D1 also regulates genes involved in lipid and glucose metabolism, mitochondrial biogenesis, and immune function.
The NR1D1 gene is located on chromosome 17q21.2 and encodes a protein composed of a DNA-binding domain with two zinc fingers and a ligand-binding domain that interacts with heme. Binding of heme modulates NR1D1 conformation and repressor activity, making it a redox-sensitive regulator of transcription. Through this mechanism, NR1D1 couples cellular metabolic state to circadian oscillations and energy balance. It directly represses key metabolic genes such as PGC-1alpha and BMAL1, thereby coordinating mitochondrial output and lipid storage cycles.
In metabolic tissues such as liver, skeletal muscle, and adipose tissue, NR1D1 acts as a gatekeeper for energy utilization. Its activation suppresses lipogenesis and promotes fatty acid oxidation. Dysregulation of NR1D1 expression has been associated with obesity, type 2 diabetes, and inflammatory disorders. Additionally, NR1D1 influences macrophage polarization and cytokine production, linking circadian control to immune homeostasis.
NR1D1 antibody is widely used in chronobiology, endocrinology, and transcriptional regulation research. It is valuable for immunoblotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to study nuclear localization, DNA binding, and protein interactions. The antibody helps elucidate NR1D1's involvement in circadian rhythm synchronization, metabolic adaptation, and disease-related transcriptional control. In cancer research, altered NR1D1 expression influences tumor metabolism and proliferation, reflecting its broader impact on cellular energy networks.
Structurally, NR1D1 forms homodimers or heterodimers with nuclear receptor corepressors and interacts with histone deacetylases to remodel chromatin. Post-translational regulation includes phosphorylation and SUMOylation, which modulate its stability and DNA-binding affinity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
