| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NR1D2 recombinant protein (Position: K56-E501) was used as the immunogen for the NR1D2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NR1D2 Antibody / Rev-erb beta / Nuclear receptor subfamily 1 group D member 2 is a anti-NR1D2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: NR1D2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, NR1D2 antibody identifies a 579-amino-acid protein that binds to Rev-erb response elements (RREs) in the promoters of target genes to repress transcription. NR1D2 interacts with the nuclear receptor co-repressor complex (NCoR/HDAC3), recruiting histone deacetylases to chromatin and silencing gene expression. It regulates genes involved in lipid synthesis, gluconeogenesis, mitochondrial function, and the circadian machinery, particularly in coordination with its paralog NR1D1 (Rev-erb alpha).
The NR1D2 gene is located on chromosome 3p24.2 and is expressed rhythmically in liver, muscle, adipose tissue, and brain. Expression oscillates in antiphase to the transcriptional activators BMAL1 and CLOCK, ensuring temporal control of metabolic and circadian genes. NR1D2 also responds to heme as a natural ligand, linking metabolic redox state to transcriptional regulation.
Pathologically, altered NR1D2 function contributes to circadian rhythm disorders, metabolic syndrome, and inflammatory diseases. Reduced activity disrupts daily metabolic cycles and leads to increased lipid accumulation and insulin resistance. Conversely, overactivation suppresses inflammatory gene expression and may offer therapeutic benefits in metabolic inflammation. Research using NR1D2 antibody supports studies in chronobiology, metabolism, and transcriptional regulation.
NR1D2 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect nuclear transcription factors.
Structurally, Nuclear receptor subfamily 1 group D member 2 contains a conserved DNA-binding domain with two zinc fingers, a ligand-binding domain for heme interaction, and a repressor interface for co-repressor complex recruitment. Its activity oscillates with diurnal rhythm, influencing both chromatin accessibility and transcriptional output. This antibody enables exploration of NR1D2's role in circadian transcriptional repression and metabolic synchronization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
