| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NR1H3 recombinant protein (Position: D11-R401) was used as the immunogen for the NR1H3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NR1H3 Antibody / Oxysterols receptor LXR-alpha is a anti-NR1H3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NR1H3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NR1H3 antibody identifies a 447-amino-acid receptor that binds oxysterol ligands, including 24(S)-hydroxycholesterol and 27-hydroxycholesterol. Upon activation, NR1H3 forms heterodimers with RXR and binds to LXR response elements in target gene promoters, stimulating transcription of ABCA1, SREBP-1c, and APOE to promote cholesterol efflux and fatty acid synthesis.
The NR1H3 gene is located on chromosome 11p11.2 and is highly expressed in liver, macrophages, and intestine. Its activity coordinates lipid handling in hepatocytes and immune cells, maintaining systemic cholesterol balance and preventing lipid accumulation. NR1H3 also modulates inflammatory gene expression by antagonizing NF-kappaB signaling.
Pathologically, dysregulation of NR1H3 contributes to atherosclerosis, fatty liver disease, and metabolic syndrome. Genetic variants in NR1H3 have been associated with autoimmune and neuroinflammatory disorders, including multiple sclerosis. Research using NR1H3 antibody supports studies in lipid regulation, transcriptional control, and metabolic disease mechanisms.
NR1H3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear receptors and transcription factors.
Structurally, Oxysterols receptor LXR-alpha contains a DNA-binding zinc finger domain and a ligand-binding domain that mediates dimerization and transcriptional activation. This antibody supports analysis of NR1H3's regulatory functions in cholesterol and lipid homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
