| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to Neuronal cell adhesion molecule protein was used as the immunogen for the NRCAM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NRCAM Antibody / Neuronal cell adhesion molecule is a anti-NRCAM Rabbit antibody Recombinant Rabbit Monoclonal clone ACOH-14 supplied in Purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: NRCAM
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone ACOH-14, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB
Biological background
During development, NRCAM contributes to neurite outgrowth and guidance by interacting with other adhesion molecules, extracellular matrix proteins, and cytoskeletal regulators. It facilitates the migration of neurons and the fasciculation of axons, ensuring proper connectivity in the developing brain and spinal cord. In mature neurons, NRCAM continues to support synaptic plasticity and neuronal communication, making it critical for both development and functional maintenance of the nervous system.
Research has linked NRCAM to multiple human diseases. Genetic studies suggest associations with autism spectrum disorders, schizophrenia, and attention deficit hyperactivity disorder, where altered cell adhesion and synaptic signaling may contribute to pathogenesis. NRCAM expression has also been implicated in cancer biology, particularly in melanoma and neuroblastoma, where it may promote tumor cell adhesion, migration, and metastasis. These findings highlight NRCAM as a multifunctional protein relevant to both neuroscience and oncology.
At the molecular level, neuronal cell adhesion molecule contains six immunoglobulin-like domains and five fibronectin type III repeats, followed by a single transmembrane region and a cytoplasmic tail. This structure enables NRCAM to form homophilic and heterophilic interactions that regulate adhesion and signaling. Through links to the actin cytoskeleton and intracellular kinases, NRCAM modulates cellular morphology, motility, and signaling responses.
The NRCAM antibody is widely used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect protein expression and localization. These applications are essential for mapping NRCAM distribution in developing and mature tissues, as well as examining its role in disease states. For researchers focused on neural connectivity, synapse biology, or tumor cell behavior, the NRCAM antibody provides a reliable detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
