| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601) was used as the immunogen for the NRF2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NRF2 Antibody / Nuclear factor erythroid 2-related factor 2 / NFE2L2 is a anti-NRF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: NRF2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, WB
Biological background
Functionally, NRF2 antibody identifies a 605-amino-acid protein that binds to DNA as a heterodimer with small Maf proteins. Under basal conditions, NRF2 is bound by KEAP1 in the cytoplasm and targeted for proteasomal degradation. Upon oxidative stress, NRF2 escapes KEAP1 repression, translocates to the nucleus, and induces genes encoding detoxification enzymes, antioxidant proteins, and drug-metabolizing enzymes such as NQO1, HO-1, and GCLM.
The NFE2L2 gene is located on chromosome 2q31.2 and is expressed in multiple tissues, with high levels in liver, lung, and kidney. NRF2 acts as a master regulator of cellular defense mechanisms, maintaining redox balance, xenobiotic metabolism, and mitochondrial function.
Pathologically, persistent NRF2 activation contributes to tumorigenesis and drug resistance, while impaired NRF2 signaling increases susceptibility to oxidative injury and chronic diseases such as neurodegeneration and fibrosis. Research using NRF2 antibody supports studies in transcriptional regulation, redox signaling, and cytoprotection.
NRF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect transcription factors involved in antioxidant defense.
Structurally, Nuclear factor erythroid 2-related factor 2 contains multiple Neh (Nrf2-ECH homology) domains that mediate DNA binding, KEAP1 interaction, and transcriptional activation. This antibody aids investigation of NRF2's dynamic regulation and its role in cellular adaptation to stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
