| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human NRXN2 was used as the immunogen for the NRXN2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NRXN2 Antibody / Neurexin 2 is a anti-NRXN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: NRXN2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB
Biological background
Structurally, Neurexin 2 exists in multiple isoforms generated by alternative promoter usage and extensive splicing, including a long alpha form and a shorter beta form. The alpha isoform contains laminin, neurexin, and sex hormone-binding globulin (LNS) domains as well as EGF-like repeats that facilitate adhesion and receptor interaction. The beta isoform retains the transmembrane and cytoplasmic PDZ-binding motifs that mediate synaptic signaling. These isoforms together ensure molecular diversity and specificity in synapse targeting and stability.
The NRXN2 antibody is widely used in neuroscience, neurodevelopmental, and molecular signaling research to study synaptic connectivity, axon guidance, and neuropsychiatric disorders. Western blot analysis detects multiple bands corresponding to the various isoforms of Neurexin 2 (ranging from 95 to 160 kilodaltons), while immunofluorescence shows punctate staining along axons and presynaptic terminals. This antibody enables characterization of neurexin distribution and expression in both developing and mature neural networks.
Functionally, Neurexin 2 regulates synapse formation by bridging pre- and postsynaptic membranes, facilitating calcium channel clustering and neurotransmitter release. Mutations or deletions in NRXN2 have been associated with autism spectrum disorder, schizophrenia, and intellectual disability, reflecting its critical role in neuronal circuit function. The NRXN2 antibody provides an essential tool for examining how neurexin-mediated adhesion and signaling shape neuronal connectivity and plasticity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
