| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human NSDHL was used as the immunogen for the NSDHL antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
NSDHL Antibody / NADP dependent steroid dehydrogenase like protein is a anti-NSDHL Rabbit antibody Recombinant Rabbit Monoclonal clone 30N01 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: NSDHL
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30N01, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
NSDHL antibody is widely applied in studies of cholesterol metabolism, embryogenesis, dermatology, and disease research. Mutations in NSDHL cause CHILD syndrome, a rare X linked disorder that results in congenital hemidysplasia, ichthyosiform erythroderma, and limb defects. By detecting NSDHL, researchers can trace how mutations impair cholesterol synthesis and lead to toxic sterol accumulation. The protein is expressed in multiple tissues with high metabolic activity, including liver, brain, and skin, making it a valuable marker for sterol pathway integrity.
Applications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays identify NSDHL expression in tissue lysates, immunohistochemistry highlights spatial distribution in liver and epidermis, and immunofluorescence reveals its localization in the endoplasmic reticulum. These methods allow researchers to connect protein expression to sterol metabolism and developmental outcomes. Because cholesterol synthesis is fundamental across organisms, NSDHL antibody has relevance in both basic biology and translational studies.
Biochemically, NSDHL functions alongside other C4 demethylation enzymes, including sterol C4 methyl oxidase and 3 beta hydroxysteroid dehydrogenase like proteins. Together they coordinate the removal of C4 methyl groups, ensuring accurate production of downstream sterols. Disruption of NSDHL activity interrupts this sequence, creating intermediates that impair membranes and signal transduction. By applying NSDHL antibody, scientists can evaluate enzyme function within the broader sterol demethylation complex.
Beyond congenital syndromes, NSDHL is increasingly studied in oncology. Cancer cells reprogram metabolism to support proliferation, and cholesterol synthesis is frequently upregulated. NSDHL expression correlates with tumor growth and resistance to certain therapies. Inhibiting sterol biosynthetic enzymes, including NSDHL, is a promising therapeutic strategy under investigation. The antibody therefore has applications in cancer metabolism research, where monitoring NSDHL may reveal vulnerabilities to metabolic intervention.
Cholesterol synthesis also intersects with neurobiology. The brain relies heavily on de novo cholesterol production, and NSDHL mutations or altered expression affect myelination and neuronal function. By detecting NSDHL with antibody based assays, researchers can study sterol metabolism in neurodevelopmental disorders and neurodegeneration. These insights extend to understanding how cholesterol imbalances influence synaptic function and cognition.
NSDHL antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
