| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NSL1 recombinant protein (Position: M1-K231) was used as the immunogen for the NSL1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NSL1 Antibody / Kinetochore-associated protein NSL1 is a anti-NSL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NSL1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NSL1 antibody identifies a 281-amino-acid nuclear and centromere-localized protein that forms part of the MIS12 complex, along with MIS12, DSN1, and PMF1. This complex acts as a central linker connecting the inner kinetochore, bound to centromeric DNA, with the outer kinetochore microtubule-binding components. NSL1 stabilizes kinetochore architecture and promotes tension-dependent microtubule attachment, which is essential for accurate chromosome alignment and segregation.
The NSL1 gene is located on chromosome 1q32.3 and encodes a coiled-coil domain-containing protein that contributes to kinetochore-spindle interactions. Through its association with the MIS12 complex, NSL1 participates in recruiting key outer kinetochore proteins such as NDC80 and KNL1, forming the KMN network that mediates microtubule attachment and spindle checkpoint signaling. Disruption of NSL1 function causes chromosome mis-segregation, aneuploidy, and mitotic arrest.
Beyond its structural role, NSL1 coordinates with spindle assembly checkpoint proteins to monitor proper kinetochore-microtubule tension. By serving as a molecular bridge between centromeric chromatin and spindle fibers, NSL1 ensures mitotic fidelity and genomic stability. Dysregulation or mutation of kinetochore components, including NSL1, contributes to chromosomal instability observed in cancer cells, highlighting its importance in tumor biology.
NSL1 antibody is widely used in cell cycle, mitosis, and chromosome segregation research. It is suitable for immunofluorescence, immunoblotting, and immunoprecipitation to study kinetochore organization and spindle checkpoint regulation. In cancer biology, this antibody supports investigations into chromosomal instability mechanisms, mitotic defects, and checkpoint adaptation. It also aids in mapping kinetochore composition across cell cycle phases.
Structurally, NSL1 contains coiled-coil motifs and conserved interaction domains that facilitate complex assembly with MIS12 and DSN1. Its function depends on dynamic phosphorylation and protein-protein interactions during metaphase and anaphase transitions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
