| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NSMAF recombinant protein (Position: K51-L868) was used as the immunogen for the NSMAF antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NSMAF Antibody / Neutral sphingomyelinase activation associated factor is a anti-NSMAF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NSMAF
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
At the cellular level, NSMAF antibody recognizes a protein localized in the cytoplasm and associated with endoplasmic reticulum membranes during receptor activation. Upon TNF-alpha or Fas ligand stimulation, NSMAF interacts with TNF receptor-associated factor 2 (TRAF2) and FAN-interacting proteins, triggering the hydrolysis of sphingomyelin into ceramide by nSMase2. Ceramide acts as a bioactive lipid second messenger that regulates apoptosis, autophagy, inflammation, and oxidative stress responses. This signaling mechanism is essential for maintaining immune homeostasis and cellular stress adaptation.
Functional studies have shown that NSMAF contributes to cell death regulation, immune signaling, and host defense mechanisms. Dysregulation of the NSMAF-nSMase-ceramide pathway has been implicated in chronic inflammatory diseases, metabolic disorders, and neurodegeneration. In the nervous system, ceramide accumulation triggered by NSMAF signaling can promote neuronal apoptosis and has been associated with diseases such as multiple sclerosis and Alzheimer's disease. Conversely, controlled activation of NSMAF supports immune responses by facilitating cytokine release and pathogen clearance. The protein may also modulate NF-kappaB signaling and contribute to receptor-mediated endocytosis.
Structurally, NSMAF consists of several WD repeat domains that create a stable platform for protein interaction with nSMase2, TRADD, and RIP1. This organization enables NSMAF to function as a scaffold for assembly of TNF receptor signaling complexes. Alternative splicing of NSMAF produces isoforms that may vary in their regulatory or tissue-specific functions. Expression is highest in immune organs such as spleen, lymph nodes, and thymus, but is also detected in brain, liver, and kidney, reflecting its broad physiological role.
Clinically, mutations or altered expression of NSMAF have been linked to immune dysregulation, susceptibility to infections, and cancer. Impaired NSMAF signaling can lead to defective ceramide synthesis and resistance to apoptosis in tumor cells. Inflammatory pathologies such as colitis and rheumatoid arthritis show altered ceramide signaling patterns involving NSMAF activation. Its involvement in lipid-mediated signaling makes it a target of interest for therapeutic interventions aiming to modulate sphingolipid metabolism and inflammatory pathways.
Immunohistochemical analysis using NSMAF antibody shows strong cytoplasmic staining in immune and epithelial tissues. The NSMAF antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
