| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NSMCE4A recombinant protein (Position: D69-Q380) was used as the immunogen for the NSMCE4A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NSMCE4A Antibody / Non-SMC element 4 homolog A is a anti-NSMCE4A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: NSMCE4A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, NSMCE4A is a 34 kilodalton nuclear protein that interacts directly with SMC5 and SMC6 through its C-terminal coiled-coil domains, forming a stable hinge component of the complex. Its N-terminal region mediates associations with additional subunits such as NSMCE1 and NSMCE2, which confer ubiquitin ligase activity and DNA damage response signaling capacity. This modular organization enables NSMCE4A to coordinate complex assembly and spatial localization at damaged DNA sites.
The NSMCE4A antibody is widely used in molecular biology, DNA repair, and cell cycle research to study chromosomal maintenance, recombination, and replication dynamics. Western blot analysis identifies a 34 kilodalton band corresponding to NSMCE4A, while immunofluorescence shows punctate nuclear staining that increases following DNA damage. This antibody provides a powerful reagent for investigating the SMC5/6 complex and its role in genome stability.
Functionally, NSMCE4A is required for homologous recombination repair, resolution of DNA replication stress, and suppression of chromosomal rearrangements. Loss of NSMCE4A disrupts complex assembly, leading to DNA double-strand break accumulation and mitotic abnormalities. The SMC5/6 complex, including NSMCE4A, also regulates the maintenance of repetitive DNA sequences and prevents unscheduled recombination at telomeres and ribosomal DNA. Aberrant NSMCE4A expression or mutation has been implicated in genome instability disorders and cancer, where defective repair pathways promote genomic rearrangements. The NSMCE4A antibody enables sensitive detection and quantification of this repair factor in mechanistic studies of DNA integrity, replication stress, and chromosome segregation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
