| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NSUN7 recombinant protein (Position: D83-Q457) was used as the immunogen for the NSUN7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NSUN7 Antibody / NOP2/Sun RNA methyltransferase family member 7 is a anti-NSUN7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NSUN7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, NSUN7 is a 721-amino-acid mitochondrial and cytoplasmic protein of approximately 80 kilodaltons containing a conserved catalytic cysteine motif and an S-adenosylmethionine (SAM)-binding domain required for methyl group transfer. It is expressed predominantly in testes, brain, and metabolic tissues, reflecting its involvement in mitochondrial gene regulation and male fertility. The enzyme localizes to the mitochondrial matrix and cytoplasmic ribonucleoprotein complexes, where it modifies tRNAs and mRNAs.
The NSUN7 antibody is widely used in epigenetics, reproductive biology, and mitochondrial research to study RNA methylation, translational regulation, and sperm function. Western blot analysis detects an 80 kilodalton band corresponding to NSUN7, while immunofluorescence reveals cytoplasmic and mitochondrial staining in germ cells and metabolic tissues. This antibody supports studies examining RNA modification-dependent regulation of gene expression.
Functionally, NSUN7 contributes to mitochondrial energy homeostasis and sperm motility by methylating specific RNA molecules required for mitochondrial translation. Mutations in NSUN7 cause male infertility due to defective sperm motility and impaired mitochondrial activity. Emerging evidence suggests that RNA methylation by NSUN7 influences cellular energy balance and oxidative stress responses beyond the reproductive system. The NSUN7 antibody provides a powerful reagent for studying RNA modification pathways, mitochondrial RNA biology, and epigenetic regulation of metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
