| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NTMT2 recombinant protein (Position: M1-S283) was used as the immunogen for the NTMT2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NTMT2 Antibody / N-terminal methyltransferase 2 is a anti-NTMT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Rat.
Key elements and design rationale
- Target: NTMT2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, NTMT2 is an approximately 32 kilodalton enzyme localized primarily to the nucleus, where it targets proteins containing an N-terminal X-Pro-Lys motif for mono-, di-, or trimethylation. It possesses a conserved methyltransferase catalytic domain that binds SAM as a methyl donor and a flexible substrate recognition pocket that determines sequence specificity. Through these enzymatic properties, NTMT2 regulates key nuclear processes, including chromatin compaction and DNA damage signaling.
The NTMT2 antibody is widely used in epigenetics, molecular biology, and proteomics research to study protein methylation, nuclear regulation, and post-translational modification networks. Western blot analysis identifies a 32 kilodalton band corresponding to NTMT2, while immunofluorescence reveals strong nuclear and nucleolar localization consistent with its methyltransferase function. This antibody provides a robust reagent for quantifying NTMT2 expression and exploring its role in gene regulation and chromatin state control.
Functionally, NTMT2 catalyzes the methylation of numerous substrates, including centromere-associated proteins and chromatin regulators, influencing their stability and recruitment to DNA-binding complexes. It works in coordination with NTMT1 to maintain proper levels of N-terminal methylation, essential for genomic stability and cell proliferation. Dysregulation of NTMT2 expression has been linked to cancer, where altered methylation affects DNA repair and transcriptional reprogramming. The NTMT2 antibody supports mechanistic studies of post-translational modification systems and their contribution to epigenetic control and cellular differentiation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
