| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NTN4 recombinant protein (Position: C?) was used as the immunogen for the NTN4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NTN4 Antibody / Netrin 4 is a anti-NTN4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: NTN4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
Functionally, NTN4 antibody identifies a 628-amino-acid protein secreted into the extracellular space, where it binds to receptors such as UNC5B, DCC, and integrins. NTN4 regulates neuronal guidance during development by controlling axonal attraction and repulsion. Beyond the nervous system, NTN4 influences angiogenesis by modulating endothelial cell proliferation and migration, acting as both a pro- and anti-angiogenic factor in different contexts. NTN4 also contributes to basement membrane organization and vascular permeability regulation.
The NTN4 gene is located on chromosome 12q22 and is expressed in brain, lung, kidney, and vascular tissues. Expression is upregulated during embryonic development, tissue regeneration, and pathological angiogenesis. NTN4 is also found in the extracellular matrix of epithelial and endothelial cells, where it interacts with laminins and collagen IV to maintain structural stability.
Pathologically, altered NTN4 expression is associated with cancer, diabetic retinopathy, and neurodevelopmental disorders. Overexpression can promote tumor growth and angiogenesis, while loss of function leads to impaired vessel formation and axonal misguidance. NTN4 acts as a context-dependent regulator of cellular adhesion, guiding both neural and vascular remodeling. Research using NTN4 antibody supports studies in extracellular matrix biology, neurodevelopment, and angiogenesis.
NTN4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted matrix proteins.
Structurally, Netrin-4 consists of a laminin N-terminal domain (LN), three laminin-type epidermal growth factor-like repeats (LE), and a C-terminal NTR domain that mediates receptor interactions. This modular organization enables NTN4 to function as both a structural matrix component and a signaling ligand. This antibody facilitates research into NTN4's roles in neural patterning, angiogenesis, and tumor microenvironment regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
