| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse NTSR2 recombinant protein (Position: Q176-E392) was used as the immunogen for the NTSR2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NTSR2 Antibody / Neurotensin receptor type 2 is a anti-NTSR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NTSR2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NTSR2 antibody identifies a 410-amino-acid seven-transmembrane receptor localized primarily in the central nervous system and peripheral tissues. NTSR2 regulates dopaminergic signaling, nociception, and gastrointestinal motility. It is distinct from NTSR1 in its lower affinity for neurotensin and its unique coupling to Gq and Gi proteins, leading to both excitatory and inhibitory cellular effects.
The NTSR2 gene is located on chromosome 2p25.1 and is highly expressed in the brainstem, hypothalamus, and spinal cord. Peripheral expression in immune cells and smooth muscle contributes to neuroimmune and cardiovascular regulation. NTSR2 activation influences neurotransmitter release, stress response, and vascular tone.
Pathologically, dysregulation of NTSR2 signaling has been associated with pain disorders, schizophrenia, addiction, and cancer progression. Its activation promotes cell migration and survival in certain tumors. Research using NTSR2 antibody supports studies in neuroscience, signal transduction, and receptor pharmacology.
NTSR2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect GPCRs and neuropeptide receptors.
Structurally, Neurotensin receptor type 2 features seven transmembrane helices typical of GPCRs, an extracellular ligand-binding region, and an intracellular tail that interacts with G proteins and arrestins. This antibody enables investigation of NTSR2's regulatory role in neurotransmission and cellular signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
