| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUDT18 recombinant protein (Position: M1-R291) was used as the immunogen for the NUDT18 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUDT18 Antibody / Nudix hydrolase 18 is a anti-NUDT18 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NUDT18
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
NUDT18 is encoded by the NUDT18 gene located on human chromosome 10q24.1. The protein contains the characteristic Nudix box motif (GX5EXEEAXE), which coordinates divalent metal ions and mediates catalysis. NUDT18 hydrolyzes substrates such as ADP-ribose and 8-oxo-dGDP, generating corresponding monophosphates and thereby removing potentially harmful oxidized nucleotide intermediates. It is localized in both cytoplasmic and mitochondrial compartments, indicating a role in protecting nucleic acid integrity in multiple cellular environments.
The NUDT18 antibody is typically used to detect a band near 35 kilodaltons on western blot, corresponding to the predicted molecular weight of the mature enzyme. Immunofluorescence analysis shows cytosolic and perinuclear localization, often associated with mitochondria-rich regions. NUDT18 expression is induced by oxidative agents and during cellular stress, suggesting a compensatory mechanism to prevent mutagenesis. Studies have shown that loss or suppression of NUDT18 increases susceptibility to oxidative DNA damage and enhances mutation frequency, linking it to cancer prevention and mitochondrial homeostasis.
Beyond its antioxidant role, NUDT18 contributes to the regulation of ADP-ribose metabolism, a process associated with signaling pathways involving poly(ADP-ribose) polymerases (PARPs) and sirtuins. By degrading ADP-ribose derivatives, NUDT18 helps reset post-translational modification cycles that regulate DNA repair and apoptosis. Dysregulation of this enzyme has been implicated in metabolic stress responses, neurodegenerative diseases, and tumor biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
