| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUDT7 recombinant protein (Position: M1-L238) was used as the immunogen for the NUDT7 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUDT7 Antibody / Peroxisomal coenzyme A diphosphatase NUDT7 is a anti-NUDT7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi, Nucleus.
Key elements and design rationale
- Target: NUDT7
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, NUDT7 antibody identifies a 282-amino-acid peroxisomal enzyme that hydrolyzes CoA, dephospho-CoA, and acyl-CoA derivatives into 3?,5?-ADP and the corresponding phosphopantetheine moiety. This activity helps regulate peroxisomal CoA levels, preventing CoA accumulation and maintaining optimal substrate availability for beta-oxidation and lipid metabolism. By modulating CoA turnover, NUDT7 indirectly influences energy metabolism, fatty acid oxidation, and reactive oxygen species detoxification.
The NUDT7 gene is located on chromosome 16p13.3 and is primarily expressed in liver and kidney, with additional expression in heart and skeletal muscle. Its activity is tightly regulated by nutritional status and metabolic signals such as fasting, reflecting its integration within energy homeostasis pathways.
Pathologically, altered NUDT7 activity can disrupt lipid metabolism and contribute to metabolic disorders. Reduced enzyme activity impairs peroxisomal CoA recycling, potentially leading to accumulation of acyl-CoA intermediates and oxidative stress. Overexpression has been linked to adaptive metabolic responses during fasting or high-fat diet conditions. Research using NUDT7 antibody supports studies in lipid metabolism, peroxisomal function, and metabolic regulation.
NUDT7 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect peroxisomal enzymes.
Structurally, Peroxisomal coenzyme A diphosphatase NUDT7 contains a conserved Nudix motif (GX5EX7REUXEEXGU) essential for divalent cation-dependent hydrolysis. The enzyme forms homodimers within peroxisomes, providing catalytic efficiency and substrate specificity. This antibody enables the study of NUDT7's role in peroxisomal metabolic control and its contribution to energy regulation in mammalian cells.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
