| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUDT8 recombinant protein (Position: M1-L236) was used as the immunogen for the NUDT8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUDT8 Antibody / Nudix hydrolase 8 is a anti-NUDT8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: NUDT8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Structurally, NUDT8 contains the conserved Nudix (nucleoside diphosphate linked to another moiety, X) motif GX5EX7REUXEEXGU, which defines the catalytic site of the Nudix hydrolase family. The enzyme hydrolyzes substrates such as CoA diphosphate derivatives, ADP-ribose, and other nucleotide intermediates. NUDT8 belongs to the mitochondrial subfamily of Nudix enzymes, which also includes NUDT9 and NUDT15. Co-localization studies confirm its mitochondrial matrix localization, consistent with roles in energy metabolism and oxidative stress defense.
Functionally, NUDT8 acts as a metabolic housekeeper by removing oxidized or excess nucleotide derivatives that can disrupt mitochondrial function. It maintains CoA and ADP-ribose homeostasis, supporting efficient energy production and mitochondrial redox balance. NUDT8 also contributes to nucleotide quality control by hydrolyzing damaged substrates generated during oxidative stress. Through this activity, it supports mitochondrial DNA replication fidelity and prevents accumulation of noncanonical nucleotides.
NUDT8 expression is developmentally regulated, increasing during mitochondrial biogenesis and metabolic activation in muscle and neuronal cells. Dysregulation of NUDT8 has been linked to metabolic disorders and mitochondrial dysfunction. Reduced enzyme activity can impair oxidative phosphorylation, while overexpression enhances resilience against oxidative damage. Pathway involvement includes CoA metabolism, NAD metabolism, and nucleotide salvage pathways.
The NUDT8 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
