| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUDT9 recombinant protein (Position: R4-L350) was used as the immunogen for the NUDT9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUDT9 Antibody / ADP-ribose pyrophosphatase is a anti-NUDT9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: NUDT9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Structurally, NUDT9 is a 350-amino-acid mitochondrial enzyme of approximately 40 kilodaltons containing a conserved Nudix box motif (GX5EXEEAXEE) that coordinates magnesium ions for catalytic hydrolysis of ADP-ribose. The enzyme localizes primarily to mitochondria and cytoplasm, where it prevents accumulation of free ADP-ribose that could perturb energy metabolism and calcium homeostasis. Its enzymatic activity helps recycle components of NAD+ metabolism and mitigate oxidative stress.
The NUDT9 antibody is widely used in biochemistry, metabolism, and mitochondrial biology research to study nucleotide turnover, ADP-ribose signaling, and cellular energy regulation. Western blot analysis detects a 40 kilodalton band corresponding to NUDT9, while immunofluorescence reveals mitochondrial and cytosolic localization. This antibody supports studies exploring enzymatic regulation of NAD+ pathways and mitochondrial nucleotide dynamics.
Functionally, NUDT9 plays a protective role against oxidative stress by regulating levels of ADP-ribose, a molecule that can activate TRPM2 channels and trigger calcium influx during stress responses. Dysregulation of NUDT9 expression affects calcium signaling, redox balance, and cell viability. Homologous enzymes and domains, such as those found in the TRPM2 channel, share structural similarity with NUDT9 and extend its functional relevance to ion channel regulation. The NUDT9 antibody provides a sensitive reagent for investigating nucleotide metabolism, mitochondrial stress adaptation, and PARP-related signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
