| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Nup107 was used as the immunogen for the NUP107 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUP107 Antibody / Nucleoporin 107 is a anti-NUP107 Rabbit antibody Recombinant Rabbit Monoclonal clone 29N68 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: NUP107
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29N68, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Nuclear pore complex protein Nup107 participates in the bidirectional transport of macromolecules, including mRNA, ribonucleoproteins, and proteins with nuclear localization or export signals. The Nup107 complex acts as a scaffold for pore assembly and contributes to nuclear envelope reformation after mitosis. Research using NUP107 antibody has revealed that disruption of this subunit leads to defective pore assembly, impaired nucleocytoplasmic trafficking, and cell cycle arrest. Its fundamental role in nuclear structure makes it indispensable for normal cell physiology.
Mutations or reduced expression of NUP107 have been linked to human disease, particularly nephrotic syndrome and premature ovarian failure. These conditions arise from defective nuclear pore assembly, underscoring the importance of this protein in development and tissue homeostasis. Studies with NUP107 antibody have also associated its dysfunction with cancers, where altered transport may affect oncogene regulation and DNA repair pathways. By examining Nup107 expression, researchers can better understand how nuclear pore biology intersects with human disease.
NUP107 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry. Western blotting reveals protein levels in dividing cells, while immunofluorescence shows localization at the nuclear envelope throughout interphase and redistribution during mitosis. Immunohistochemistry highlights expression in kidney, ovary, and other tissues affected by Nup107 mutations. These applications demonstrate the utility of NUP107 antibody in diverse cell biology and pathology research.
By supplying validated NUP107 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
