| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUP160 recombinant protein (Position: E880-K1364) was used as the immunogen for the NUP160 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUP160 Antibody / Nuclear pore complex protein Nup160 is a anti-NUP160 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: NUP160
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Nuclear pore complex protein Nup160 contributes to the assembly and stability of the Nup107-160 complex, which includes Nup107, Nup133, Nup96, and Sec13. It localizes to both the cytoplasmic and nucleoplasmic faces of the nuclear envelope and interacts with importins, exportins, and Ran GTPase to regulate nuclear transport. The NUP160 antibody enables detection of this scaffold protein in isolated nuclei and cultured cells, revealing how changes in NPC composition affect molecular trafficking.
Beyond transport, Nuclear pore complex protein Nup160 plays key roles in mitosis. During cell division, the Nup107-160 complex redistributes to kinetochores, where it assists in spindle assembly and chromosome segregation. The NUP160 antibody supports visualization of these dynamic localization patterns, helping clarify the interface between nuclear pore biology and mitotic regulation. Disruption of NUP160 expression or function leads to nuclear envelope defects, delayed cell-cycle progression, and genomic instability.
Mutations in NUP160 have been linked to steroid-resistant nephrotic syndrome and immune dysregulation, reflecting the importance of intact nuclear transport in tissue homeostasis. The NUP160 antibody is used in studies of nuclear pore-related diseases, including autoimmune and neurodegenerative conditions. Reduced expression or structural alteration of Nup160 can also influence viral nuclear import and cellular stress responses.
The NUP160 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, yielding strong nuclear envelope staining consistent with pore complex localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
