| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUP88 recombinant protein (Position: E91-K584) was used as the immunogen for the NUP88 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUP88 Antibody / Nuclear pore complex protein Nup88 / Nucleoporin 88 is a anti-NUP88 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: NUP88
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Functionally, NUP88 antibody identifies a 741-amino-acid nucleoporin localized to the cytoplasmic face of the nuclear pore complex. NUP88 forms a subcomplex with NUP214 and NUP62 that assists in nuclear export of ribonucleoproteins and protein cargoes. It is also involved in spindle assembly and chromosome segregation during mitosis, highlighting its dual role in transport and cell cycle control.
The NUP88 gene is located on chromosome 17p13.1 and is ubiquitously expressed in proliferating cells. Its expression is dynamically regulated during mitosis and stress responses. NUP88�s proper function ensures balanced nucleocytoplasmic transport and mitotic progression, while its mislocalization disrupts nuclear integrity.
Pathologically, overexpression of NUP88 has been linked to several cancers, including breast, colorectal, and hepatocellular carcinoma, where it promotes nuclear transport of oncogenic factors. Mutations in NUP88 cause neuromuscular and developmental disorders such as fetal akinesia deformation sequence. Research using NUP88 antibody supports studies in nuclear transport, mitosis, and tumor biology.
NUP88 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear pore complex proteins.
Structurally, Nuclear pore complex protein Nup88 contains coiled-coil domains that mediate interaction with other nucleoporins and a C-terminal alpha-helical region that anchors it within the nuclear envelope. This antibody aids in analyzing NUP88's role in nuclear transport and mitotic regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
