| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NUP98 recombinant protein (Position: H728-I1787) was used as the immunogen for the NUP98 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUP98 Antibody / Nucleoporin 98 is a anti-NUP98 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: NUP98
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Nup98 contains FG (phenylalanine-glycine)-repeat domains that form flexible meshwork structures facilitating selective nucleocytoplasmic transport. The C-terminal region anchors to the NPC scaffold via interactions with other nucleoporins such as Nup96 and Nup88. Beyond its classical transport role, Nup98 shuttles between the nuclear envelope and nucleoplasm, where it binds transcriptional regulators, influencing developmental and immune response genes. It also participates in RNA export, mRNA surveillance, and mitotic spindle assembly.
The NUP98 antibody is widely used in cell biology, oncology, and molecular genetics research to study nuclear transport, chromatin organization, and cell cycle regulation. Western blot analysis identifies a 98 kilodalton band corresponding to Nup98, while immunofluorescence shows punctate staining at the nuclear envelope and within nucleoplasmic foci. This antibody supports studies examining NPC function, transcriptional regulation, and nuclear architecture integrity.
Translocations involving NUP98 are recurrent in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), producing oncogenic Nup98 fusion proteins such as NUP98-HOXA9 and NUP98-NSD1. These fusions retain the FG-repeat domain of Nup98 but acquire transcriptional activation capabilities from their fusion partners, leading to deregulated gene expression and leukemogenesis. The NUP98 antibody provides a reliable tool for studying such fusion proteins and their oncogenic mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
