| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NUPR1 was used as the immunogen for the NUPR1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NUPR1 Antibody / Nuclear protein 1 is a anti-NUPR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: NUPR1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS
Biological background
Structurally, NUPR1 is a 100-amino-acid protein of approximately 9 kilodaltons lacking defined secondary structure, which enables flexible interactions with DNA and chromatin-modifying enzymes. It acts as a transcriptional cofactor rather than a classical DNA-binding protein, partnering with p300, SMADs, and histone modifiers to influence transcriptional responses. NUPR1 localizes predominantly to the nucleus but can shuttle between compartments depending on stress conditions and post-translational modifications.
The NUPR1 antibody is widely used in oncology, stress biology, and transcription research to study adaptive gene regulation and tumor cell survival. Western blot analysis detects a 9 kilodalton band corresponding to NUPR1, while immunofluorescence demonstrates strong nuclear localization that increases following stress induction. This antibody enables analysis of NUPR1 expression during oxidative and metabolic stress and its contribution to stress-responsive transcription networks.
Functionally, NUPR1 promotes survival under cytotoxic conditions by regulating genes involved in autophagy and anti-apoptotic pathways. It acts downstream of ATF4 and CHOP in the endoplasmic reticulum stress response and influences cell fate decisions by modulating p53-dependent transcription. In cancer, overexpression of NUPR1 supports tumor growth, resistance to chemotherapy, and epithelial-mesenchymal transition (EMT). Conversely, NUPR1 depletion sensitizes tumor cells to oxidative damage and metabolic inhibitors. The NUPR1 antibody provides a key research tool for exploring stress signaling, transcriptional plasticity, and tumor adaptability.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
