| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NUSAP1 was used as the immunogen for the NUSAP1 antibody. |
| Isotype | |
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Overview
NUSAP1 Antibody / Nucleolar and spindle-associated protein 1 is a anti-NUSAP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.
Key elements and design rationale
- Target: NUSAP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS
Biological background
Functionally, NUSAP1 antibody identifies a 495-amino-acid nuclear protein that localizes to the mitotic spindle and spindle poles. NUSAP1 binds microtubules through its C-terminal domain and bundles them into stable arrays, promoting bipolar spindle formation. It also interacts with chromatin components, including DNA and nucleolar proteins, to coordinate chromosome condensation and segregation during metaphase and anaphase.
The NUSAP1 gene, located on chromosome 15q15.1, encodes a cell cycle-regulated protein that peaks in expression during G2/M phase and is degraded after mitosis. NUSAP1 is phosphorylated by CDK1, controlling its localization and activity. It functions in concert with kinesin and dynein motors, contributing to spindle microtubule dynamics and mitotic checkpoint signaling.
Pathologically, elevated NUSAP1 expression is a hallmark of proliferating cells and various cancers, including breast, prostate, and lung carcinoma. Its overexpression correlates with increased tumor aggressiveness and poor prognosis, reflecting enhanced proliferative capacity. Conversely, NUSAP1 depletion leads to spindle defects, mitotic arrest, and genomic instability. Research with NUSAP1 antibody provides insight into cell division mechanisms and cancer cell cycle regulation.
NUSAP1 antibody is suitable for use in western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindles and cell cycle progression.
Structurally, NUSAP1 contains a coiled-coil domain, a microtubule-binding motif, and a DNA-binding region, which together mediate its dual role in chromatin attachment and microtubule bundling. This antibody supports exploration of NUSAP1�s role as a spindle scaffold that integrates chromosomal and cytoskeletal control during mitosis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
