| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NYAP2 recombinant protein (Position: H83-L617) was used as the immunogen for the NYAP2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NYAP2 Antibody / Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2 is a anti-NYAP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: NYAP2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, NYAP2 antibody identifies a 1,228-amino-acid neuronal protein that interacts with IRSp53 and PI3K subunits to transmit signals from axon guidance receptors such as neuregulin and contactin-associated proteins. NYAP2 participates in a signaling cascade involving Crk-associated substrate (CAS) and small GTPases Rac1 and Cdc42, controlling actin filament organization and neuronal polarity. It localizes to axons and growth cones, where it modulates cytoskeletal remodeling during neuronal wiring and plasticity.
The NYAP2 gene is located on chromosome 2q37.3 and is selectively expressed in brain regions including cortex, hippocampus, and cerebellum. Its expression peaks during neuronal differentiation and synapse formation, aligning with its role in neurodevelopment and synaptic function.
Pathologically, altered NYAP2 expression or mutation disrupts neuronal connectivity and has been associated with neurodevelopmental disorders such as autism spectrum disorder and intellectual disability. Dysregulation of NYAP2-mediated PI3K signaling may also contribute to synaptic dysfunction in neurodegenerative diseases. Research using NYAP2 antibody supports studies in neuronal signaling, actin cytoskeleton regulation, and brain development.
NYAP2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect neuronal adaptor proteins.
Structurally, Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 2 contains multiple proline-rich motifs, SH3-binding regions, and tyrosine phosphorylation sites that enable dynamic interactions with signaling complexes. Its modular design allows integration of membrane receptor signals with intracellular actin regulatory machinery. This antibody facilitates exploration of NYAP2's function in neuronal network formation and synaptic communication.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
