| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Oas2 recombinant protein (Position: H35-K742) was used as the immunogen for the Oas2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Oas2 Antibody / 2-5-Oligoadenylate synthetase 2 is a anti-OAS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: OAS2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, OAS2 is a 113 kDa protein containing two functional OAS catalytic domains, allowing higher polymerization activity than other family members such as OAS1. It belongs to the 2'-5'-oligoadenylate synthetase family of nucleotidyltransferases, characterized by a conserved polymerase fold and dsRNA-binding motifs. OAS2 localizes to the cytoplasm, often associating with ribosomes and endoplasmic reticulum membranes where viral replication typically occurs.
Functionally, OAS2 acts as a critical antiviral effector enzyme in interferon-stimulated pathways. Upon recognition of viral RNA, OAS2 catalyzes ATP polymerization into 2-5A molecules that bind and activate latent RNase L. This leads to RNA cleavage, apoptosis of infected cells, and amplification of antiviral signaling. OAS2 also interacts with pattern recognition receptors such as MDA5 and RIG-I, coordinating interferon response cascades. In addition to antiviral defense, OAS2 influences immune regulation, apoptosis, and inflammatory cytokine expression.
OAS2 expression is strongly induced by type I and type III interferons. Genetic variants in OAS2 have been associated with altered susceptibility to viral infections including SARS-CoV-2, hepatitis C, and West Nile virus. Dysregulation of OAS2 can contribute to autoimmune disorders through excessive RNA degradation. Pathway associations include interferon signaling, RNA decay, and innate immune activation. During development, OAS2 expression in immune progenitors supports early antiviral competence.
The OAS2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
