| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human OAZ1 was used as the immunogen for the OAZ1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
OAZ1 Antibody / Ornithine decarboxylase antizyme 1 is a anti-OAZ1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: OAZ1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
Functionally, OAZ1 antibody identifies a 228-amino-acid cytoplasmic protein that binds to and inhibits the enzyme ODC, preventing the conversion of ornithine into putrescine, the first step in polyamine biosynthesis. OAZ1 also targets ODC for ubiquitin-independent proteasomal degradation, ensuring rapid turnover of the enzyme. Additionally, OAZ1 regulates polyamine transport across the plasma membrane by binding to polyamine importers and blocking uptake. These dual inhibitory actions maintain tight control of intracellular polyamine concentrations.
The OAZ1 gene is located on chromosome 19p13.3 and is expressed in nearly all tissues, with high levels in liver, brain, and reproductive organs. OAZ1 translation is uniquely regulated by a +1 ribosomal frameshifting mechanism triggered by elevated polyamine concentrations, creating an autoregulatory feedback loop that modulates its own synthesis in response to metabolic conditions.
Pathologically, dysregulation of OAZ1 contributes to cancer, neurodegeneration, and reproductive disorders. Reduced OAZ1 activity leads to elevated polyamine levels that promote uncontrolled cell proliferation, while overexpression can suppress tumor growth by restricting polyamine availability. In the nervous system, OAZ1 influences synaptic plasticity and neuronal differentiation. Research using OAZ1 antibody supports studies in metabolic regulation, cancer biology, and neurophysiology.
OAZ1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect polyamine regulators.
Structurally, Ornithine decarboxylase antizyme 1 consists of a conserved ODC-binding region that mediates enzyme inhibition and a C-terminal domain required for proteasomal targeting. The protein's structure allows it to act both as an inhibitor and degradation adaptor without requiring ubiquitin conjugation. This antibody enables detailed investigation of OAZ1's role in polyamine metabolism, feedback control, and tumor suppression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
