| Field | Specification |
|---|---|
| Target | |
| Alternative names | LEPR; DB; OBR; Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD antigen CD295 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Ob-R (phospho Tyr1141) |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010],
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Ob-R (phospho Tyr1141). It is validated for Western Blot, ELISA.
Validated Applications
| Applications | Western Blot, ELISA |
|---|---|
| Western Blot | 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Ob-R (phospho Tyr1141)
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Ob-R (phospho Tyr1141) |
| Concentration | 1 mg/mL |
| Observed Band | 132kD |
| Subcellular Location | Cell membrane ; Single-pass type I membrane protein . Basolateral cell membrane .; [Isoform E]: Secreted . |
| Swiss-Prot | P48357 |
| Gene ID | 3953 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Ob-R (phospho Tyr1141) research from ELK Biotechnology, or contact us for alternative formats.
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