| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human OGFOD3 recombinant protein (Position: D67-H311) was used as the immunogen for the OGFOD3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OGFOD3 Antibody / 2-Oxoglutarate and iron-dependent oxygenase domain-containing protein 3 is a anti-OGFOD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: OGFOD3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
OGFOD3 is expressed in most tissues, with notable enrichment in the brain, testis, and liver. Localization studies show nuclear distribution with occasional cytoplasmic presence, suggesting roles in both nuclear signaling and translational control. Comparative genomics indicates that OGFOD3 shares structural similarity with OGFOD1, an enzyme known to hydroxylate ribosomal protein RPS23, implying potential overlap in function. The OGFOD3 antibody is used to investigate dioxygenase-mediated post-translational modifications and the cellular response to oxidative or metabolic stress.
Although less characterized than its paralogs, OGFOD3 has been linked to cellular stress pathways and protein biosynthesis. Studies show that depletion of OGFOD3 alters ribosomal activity and activates integrated stress response signaling. In cancer research, OGFOD3 expression is dysregulated in several tumor types, including lung and colon carcinomas, where it may influence adaptation to hypoxia or nutrient deprivation. The OGFOD3 antibody allows researchers to detect expression changes under stress conditions and to localize the protein within cells using immunocytochemical or immunofluorescent assays. Western blot analysis identifies a 38 kilodalton band corresponding to the full-length protein.
In the context of oxygen sensing and translational control, OGFOD3 may coordinate with other 2OG oxygenases to maintain homeostasis during metabolic shifts. It potentially affects protein synthesis by modifying translation factors or ribosomal subunits, modulating global translational output in response to stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
