| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human OMA1 recombinant protein (Position: K256-H483) was used as the immunogen for the OMA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OMA1 Antibody / Overlapping with the m-AAA protease 1 is a anti-OMA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria in cytoplasm, nucleus.
Key elements and design rationale
- Target: OMA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
OMA1 is encoded by the OMA1 gene located on human chromosome 1p32.2. It encodes an integral membrane protein of the inner mitochondrial membrane, with its catalytic domain facing the intermembrane space. The protein contains a conserved HEXXH metalloprotease motif required for zinc ion coordination and proteolytic activity. OMA1 exists in inactive and active forms that are regulated by mitochondrial potential and proteolytic turnover. Activation leads to processing of long isoforms of OPA1 into short forms, promoting mitochondrial fragmentation during stress.
The OMA1 antibody is valuable for investigating mitochondrial stress pathways and proteolytic processing events. Western blot analysis typically detects bands near 60-65 kDa, corresponding to the precursor and active forms of OMA1. In immunocytochemistry, the antibody reveals punctate mitochondrial staining, confirming localization within the inner membrane network. Functional studies using OMA1-deficient cells demonstrate its essential role in maintaining mitochondrial morphology and preventing accumulation of dysfunctional organelles. Loss of OMA1 activity has been associated with neurodegeneration, cardiomyopathy, and metabolic syndromes due to impaired mitochondrial adaptation.
OMA1 acts in concert with other mitochondrial proteases, including YME1L1 and PARL, to fine-tune the balance between fusion and fission events. It also participates in the mitochondrial unfolded protein response and contributes to stress-induced apoptosis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
