| Field | Specification |
|---|---|
| Product Type | |
| Storage |
OneScript® Hot Reverse Transcriptase is a thermostable, mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) that can efficiently reverse transcribe low-abundance or partially degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. It possesses genetic modifications to abolish RNase H activity to achieve thermal stability, allowing for high processivity and sensitivity that enable rapid synthesis of full-length cDNA fragments in a fraction of the time required by leading competitors.
abm is the only company in the world to have a thermostable reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC. This is the enzyme of choice for daily or demanding RNA reverse transcription. OneScript® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Product Features:
- Thermostable from 60-72°C/140-161°F
- Transcribes degraded samples and resists inhibitors/contaminants
- Provides high cDNA yields from difficult or ultra-low RNA samples
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| OneScript® Hot Reverse Transcriptase | 100 rxn (100 µl) |
| 5X RT Buffer | 400 µl |
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ISO 13485:2016 MDSAP Certified |
|
This product is equivalent to RScript Hot Reverse Transcriptase (Cat. No. G593EU). |
| Specification | Value |
|---|---|
| Enzyme Type | Reverse Transcriptase |
| Format | Enzyme Only |
| RT Origin | M-MLV |
| Storage Conditions | Store at -20ºC. |
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
Store the synthesized first-strand cDNA at -20°C for long-term use.
Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield: Check RNA integrity with gel electrophoresis or a BioAnalyzer, aiming for a minimum A260/A280 ratio of 1.7 or a RIN above 8. Clean the RNA using ethanol precipitation or lithium chloride to remove contaminants. Purify the RNA with extraction kits or phenol/chloroform to remove proteins. Increase RNA input, especially for low-abundance samples.
We recommend using 1 ng to 2 μg of RNA per reaction.
Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
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Camas Carangui, L. I., & Chabla Morquecho, N. S. (2024). Efecto del ciclo lunar en algunas características morfológicas y funcionales del ovario en cobayas (Cavia porcellus). http://dspace.ucuenca.edu.ec/handle/123456789/44942
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Sun, Z., Geng, W., Ren, B., Zhao, B., Liu, P., & Zhang, J. (2022). Low photosynthetic rate under low light stress inhibited sucrose distribution and transportation to grain. BioRxiv, 2022-08. https://doi.org/10.1101/2022.08.02.502494
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