| Field | Specification |
|---|---|
| Product Type | |
| Storage |
OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus (M-MLV) Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.
OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.
Product Features:
- High thermostability at 50-55°C/122-131°F
- Synthesize 15 kb RNA Templates
- 10-15 minutes reaction time
- OneScript® Plus cDNA Synthesis Kit (Cat. No. G236) also available
- Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| OneScript® Plus Reverse Transcriptase | 100 rxn (100 µl) |
| 5X RT Buffer | 400 µl |
 |
ISO 13485:2016 MDSAP Certified |
|
This product is equivalent to RScript Plus Reverse Transcriptase (Cat. No. G237EU). |
| Specification | Value |
|---|---|
| Enzyme Type | Reverse Transcriptase |
| Format | Enzyme Only |
| RT Origin | M-MLV |
| Storage Conditions | Store at -20°C. |
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
Store the synthesized first-strand cDNA at -20°C for long-term use.
Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield: Check RNA integrity with gel electrophoresis or a BioAnalyzer, aiming for a minimum A260/A280 ratio of 1.7 or a RIN above 8. Clean the RNA using ethanol precipitation or lithium chloride to remove contaminants. Purify the RNA with extraction kits or phenol/chloroform to remove proteins. Increase RNA input, especially for low-abundance samples.
We recommend using 1 ng to 2 μg of RNA per reaction.
Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Chaudhuri E; Dash S; Balasubramaniam M et al.. The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b. The Journal of biological chemistry. 2020;295:5081-5094. DOI
- Gallardo CM; Wang S; Montiel-Garcia DJ et al.. MrHAMER yields highly accurate single molecule viral sequences enabling analysis of intra-host evolution. Nucleic acids research. 2021;49:e70. DOI
- Han J; Zuo J; Zhang X et al.. TRIM29 is differentially expressed in colorectal cancers of different primary locations and affects survival by regulating tumor immunity based on retrospective study and bioinformatics analysis. Journal of gastrointestinal oncology. 2022;13:1132-1151. DOI
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