| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human OPN3 was used as the immunogen for the OPN3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
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Overview
OPN3 Antibody / Opsin 3 is a anti-OPN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: OPN3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF
Biological background
Functionally, OPN3 antibody identifies a 402-amino-acid seven-transmembrane GPCR that binds retinal chromophore and activates intracellular signaling pathways in response to light or metabolic cues. OPN3 couples to heterotrimeric G proteins, influencing cyclic nucleotide levels, calcium signaling, and other downstream responses. Its activity extends beyond traditional photoreception, integrating environmental light signals into physiological regulation of circadian rhythm, metabolism, and hormone secretion.
The OPN3 gene is located on chromosome 1q43 and encodes a protein expressed in brain, skin, testis, and adipose tissue. OPN3 is localized primarily in cell membranes and intracellular vesicles, suggesting context-dependent signaling functions. In the brain, it may modulate neuronal excitability and metabolic responses to light exposure. In skin, OPN3 contributes to phototransduction in melanocytes and keratinocytes, influencing pigmentation and UV response.
Emerging evidence implicates OPN3 in non-visual light sensing that affects circadian entrainment, energy homeostasis, and mood regulation. It may act as a photoreceptor for near-infrared or blue light, mediating cellular adaptation to environmental illumination. In metabolic tissues, OPN3 modulates lipid metabolism and thermogenesis, linking light exposure to systemic energy balance. OPN3 has also been studied for its potential role in reproductive physiology and skin photobiology.
OPN3 antibody is widely used in photoreceptor biology, neuroendocrinology, and GPCR signaling research. It is suitable for immunofluorescence, western blotting, and immunohistochemistry to detect OPN3 localization in neural, skin, and endocrine tissues. This antibody supports research into light-mediated signaling, opsin evolution, and photoregulated physiology. In biomedical studies, OPN3 serves as a molecular link between light sensing and metabolic control.
Structurally, OPN3 exhibits the canonical GPCR topology with seven transmembrane helices, an extracellular retinal-binding lysine, and cytoplasmic domains that interact with G proteins.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
