| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human OPN5 was used as the immunogen for the OPN5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OPN5 Antibody / Opsin 5 / Neuropsin is a anti-OPN5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: OPN5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF
Biological background
OPN5 is expressed in brain regions such as the hypothalamus and preoptic area, as well as in peripheral tissues including retina, cornea, skin, and reproductive organs. It functions as a UV-sensitive photoreceptor regulating local circadian oscillators and hormone secretion. In the eye, OPN5 contributes to light entrainment of retinal and non-retinal cells, while in the skin and reproductive tissues, it influences seasonal and environmental light-dependent physiology. The receptor's activation triggers G protein signaling that modulates cyclic AMP levels, gene expression, and ion channel activity.
The OPN5 antibody is a valuable tool for neurobiology, circadian rhythm, and sensory physiology research. Western blot analysis detects a 40 kilodalton band corresponding to Opsin-5, while immunofluorescence and immunohistochemistry reveal membrane localization in neuronal and epithelial tissues. This antibody supports the study of light-dependent signaling outside the visual system and enables mapping of Opsin-5 expression across tissues.
Dysregulation of OPN5 signaling has been linked to altered circadian rhythms, hormonal imbalances, and impaired photic entrainment. In animal models, OPN5 influences melatonin secretion and reproductive seasonality, connecting environmental lighting cues to endocrine function. The OPN5 antibody facilitates the exploration of these mechanisms by providing specific detection of Opsin-5 in experimental systems.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
