| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human OSBPL2 recombinant protein (Position: E6-Y480) was used as the immunogen for the OSBPL2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OSBPL2 Antibody / Oxysterol-binding protein-like 2 is a anti-OSBPL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: OSBPL2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, FACS, ELISA
Biological background
Functionally, OSBPL2 antibody identifies a 916-amino-acid protein containing an N-terminal pleckstrin homology (PH) domain for membrane targeting and a C-terminal OSBP-related domain (ORD) that binds sterols and phosphatidylinositol phosphates. OSBPL2 functions as a lipid exchanger, shuttling cholesterol and phosphatidylserine between the endoplasmic reticulum and plasma membrane. Through this exchange, it regulates lipid signaling and membrane composition critical for endocytosis and vesicle formation.
The OSBPL2 gene is located on chromosome 20q13.12 and is expressed in a variety of tissues, including liver, brain, and muscle. OSBPL2 contributes to intracellular cholesterol homeostasis by facilitating lipid equilibration across membrane compartments. It interacts with small GTPases such as Rab7 and Arf1 to coordinate lipid trafficking with vesicular transport. Dysregulation of OSBPL2 affects lipid distribution, leading to membrane stress, endosomal dysfunction, and altered cellular signaling.
In lipid metabolism, OSBPL2 acts as a sensor for oxysterols and phosphoinositides, integrating lipid status with signaling pathways such as PI3K/AKT and MAPK. It also modulates the activity of lipid-metabolizing enzymes and influences the localization of phosphatidylinositol 4-phosphate at membrane contact sites. Mutations in OSBPL2 are associated with autosomal dominant hearing loss, indicating its role in membrane stability and ion channel regulation in auditory hair cells.
OSBPL2 antibody is widely used in membrane biology, metabolism, and vesicle trafficking research. It is suitable for immunoblotting, immunofluorescence, and subcellular fractionation studies to examine OSBPL2 localization and function. This antibody is valuable for exploring mechanisms of lipid sensing and non-vesicular transport between organelles. In metabolic and cell signaling studies, OSBPL2 antibody aids in understanding sterol regulation and membrane homeostasis.
Structurally, OSBPL2 features conserved lipid-binding motifs within its ORD domain and multiple targeting sequences that direct it to membrane contact sites. It can form dimers or higher-order assemblies that facilitate lipid transfer across closely apposed membranes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
