| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived rat Osm recombinant protein (Position: E55-D158) was used as the immunogen for the Osm antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Osm Antibody / Oncostatin M is a anti-Osm Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: Osm
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, OSM antibody identifies a 252-amino-acid cytokine produced primarily by activated T cells, macrophages, and monocytes. Upon secretion, OSM binds to receptor complexes composed of gp130 and OSMRbeta or LIFRbeta, initiating downstream phosphorylation of STAT3 and STAT5 transcription factors. These pathways promote expression of acute-phase proteins, matrix metalloproteinases, and cell differentiation genes. OSM plays critical roles in inflammation, angiogenesis, and wound repair by modulating fibroblast proliferation, endothelial activation, and extracellular matrix production.
The OSM gene is located on chromosome 22q12.2 and is broadly expressed in immune, endothelial, and stromal cells under inflammatory or injury conditions. Expression increases in response to IL1beta, TNFalpha, and lipopolysaccharides, linking OSM production to innate and adaptive immune responses. OSM participates in crosstalk between immune cells and tissue-resident fibroblasts, coordinating local inflammatory responses and tissue regeneration.
Pathologically, dysregulated OSM signaling contributes to chronic inflammation, fibrosis, and cancer. Elevated levels are associated with rheumatoid arthritis, atherosclerosis, and multiple sclerosis. In cancer, OSM exhibits context-dependent effects, acting as both a tumor suppressor and promoter by influencing cell proliferation and metastasis. Research using OSM antibody supports studies in cytokine signaling, inflammatory disease, and tumor biology.
OSM antibody is validated for ELISA, western blotting, and immunohistochemistry to detect cytokines in immune and stromal contexts.
Structurally, Oncostatin M contains a four-helix bundle cytokine fold stabilized by disulfide bridges and glycosylation sites essential for receptor binding and stability. The C-terminal domain interacts with gp130-containing receptor complexes to initiate signal transduction. This antibody enables detailed analysis of OSM's role in immune signaling, cytokine regulation, and tissue repair.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
