| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human OSTM1 was used as the immunogen for the OSTM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OSTM1 Antibody / Osteopetrosis-associated transmembrane protein 1 is a anti-OSTM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: OSTM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF
Biological background
OSTM1 contains a single transmembrane domain and a large luminal glycoprotein region that protects lysosomal membranes from degradation. It plays a dual role as a chaperone for CLCN7 and as a structural component of lysosomal and endosomal membranes. In addition to skeletal tissues, OSTM1 is expressed in neurons and immune cells, where it contributes to lysosomal biogenesis and degradation of cellular material. Its deficiency leads to impaired lysosomal trafficking, abnormal autophagy, and neurodegeneration.
The OSTM1 antibody is widely used in bone biology, lysosomal function, and neurodegeneration research to study protein trafficking, osteoclast activity, and lysosomal integrity. Western blot analysis identifies a 40-50 kilodalton band corresponding to glycosylated OSTM1, while immunofluorescence reveals punctate lysosomal localization that colocalizes with LAMP1 and CLCN7. This antibody supports detailed studies of lysosomal acidification, bone remodeling, and cellular degradation pathways.
Beyond osteoclast biology, OSTM1 influences autophagic flux and neuronal survival through regulation of lysosomal enzyme maturation and pH balance. The OSTM1 antibody provides an effective tool for exploring the cellular mechanisms underlying osteopetrosis, lysosomal storage disorders, and neurodegenerative diseases.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
