| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human OTUD3 recombinant protein (Position: E51-I398) was used as the immunogen for the OTUD3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OTUD3 Antibody / OTU domain-containing protein 3 is a anti-OTUD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: OTUD3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC, IF, ELISA
Biological background
Structurally, OTUD3 contains an OTU catalytic domain responsible for hydrolyzing lysine-linked ubiquitin chains, particularly K63 and K48 linkages, which regulate protein turnover and signal transduction. It stabilizes tumor suppressor p53 by removing ubiquitin moieties added by MDM2 and promotes the degradation of AKT to restrain oncogenic signaling. Through these opposing actions, OTUD3 maintains a balance between proliferation and apoptosis in response to cellular stress.
The OTUD3 antibody is used in cancer biology, proteostasis, and signal transduction research to study ubiquitin signaling, protein turnover, and stress regulation. Western blot analysis identifies a 45 kilodalton band corresponding to OTUD3, while immunofluorescence shows cytoplasmic and nuclear localization depending on cellular state. This antibody supports the investigation of deubiquitination mechanisms and ubiquitin-mediated signaling networks.
Alterations in OTUD3 expression are associated with tumorigenesis, neurodegeneration, and inflammatory diseases. Overexpression of OTUD3 stabilizes p53 and enhances apoptosis, while loss of expression correlates with increased AKT activity and cancer progression. The OTUD3 antibody provides a validated tool for examining these regulatory mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
