| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human OTUD4 recombinant protein (Position: E52-T1114) was used as the immunogen for the OTUD4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
OTUD4 Antibody / OTU domain-containing protein 4 is a anti-OTUD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: OTUD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Structurally, OTUD4 contains an OTU catalytic domain with conserved cysteine and histidine residues required for thiol protease activity. It also possesses multiple ubiquitin-binding motifs that enable recognition of specific ubiquitin linkages, conferring substrate selectivity. OTUD4 localizes to both cytoplasm and nucleus, where it modulates key signaling proteins involved in stress response, interferon signaling, and genome maintenance.
The OTUD4 antibody is widely used in ubiquitin signaling, oncology, and immunology research to study protein degradation, stability, and DNA repair. Western blot analysis identifies an approximately 130 kilodalton band corresponding to OTUD4, while immunofluorescence reveals both cytoplasmic and nuclear punctate localization, consistent with its regulatory role in ubiquitin metabolism. This antibody enables detection of dynamic changes in ubiquitin-modifying enzymes during stress and immune signaling.
Functionally, OTUD4 stabilizes DNA repair proteins such as KU80 and RPA1, promoting efficient double-strand break repair. It also regulates antiviral responses by modulating the ubiquitination of MAVS and other interferon signaling components, thereby balancing antiviral defense and immune homeostasis. Loss or mutation of OTUD4 impairs genome integrity and contributes to increased sensitivity to genotoxic stress. In cancer, dysregulated OTUD4 expression affects tumor cell proliferation and chemoresistance by altering protein turnover and checkpoint signaling. The OTUD4 antibody provides an essential tool for exploring deubiquitination pathways and their role in cell signaling and genomic maintenance.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
