| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human P3H2 recombinant protein (Position: E106-R494) was used as the immunogen for the P3H2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
P3H2 Antibody / Prolyl 3-hydroxylase 2 is a anti-P3H2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, Golgi, Nucleus.
Key elements and design rationale
- Target: P3H2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
P3H2 localizes to the lumen of the endoplasmic reticulum, where it operates as part of a multi-enzyme complex with cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). This complex ensures proper hydroxylation and folding of procollagen molecules prior to secretion. The enzymatic reaction requires Fe2+, 2-oxoglutarate, and ascorbate as cofactors. Loss of P3H2 activity leads to underhydroxylated collagen and defects in connective tissue organization.
The P3H2 antibody is used in extracellular matrix, connective tissue, and skeletal biology research to study collagen modification and protein maturation. Western blot analysis detects a 90 kilodalton band corresponding to P3H2, while immunofluorescence reveals reticular ER localization consistent with its role in collagen biosynthesis. This antibody allows characterization of collagen-modifying enzymes and post-translational hydroxylation mechanisms.
Altered expression or mutation of P3H2 has been implicated in connective tissue disorders, osteogenesis imperfecta, and fibrosis, where abnormal collagen hydroxylation compromises structural integrity. The P3H2 antibody is an essential reagent for exploring collagen maturation, matrix assembly, and disease-associated remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
