| Field | Specification |
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| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human P4HA1 was used as the immunogen for the P4HA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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Overview
P4HA1 Antibody / Prolyl 4-hydroxylase subunit alpha 1 is a anti-P4HA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: P4HA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF
Biological background
Functionally, P4HA1 antibody identifies a 534-amino-acid enzyme localized to the endoplasmic reticulum lumen, where it associates with prolyl 4-hydroxylase beta subunits to form the active tetrameric enzyme. P4HA1 catalyzes the post-translational modification of proline to 4-hydroxyproline, a critical step in collagen maturation that stabilizes the helical structure.
The P4HA1 gene is located on chromosome 10q22.1 and is expressed in fibroblasts, chondrocytes, and osteoblasts. Its expression increases during wound healing, tissue remodeling, and hypoxia. P4HA1 activity is co-regulated with other collagen synthesis enzymes and is sensitive to oxygen and ascorbate availability.
Pathologically, overexpression of P4HA1 has been linked to fibrosis, tumor progression, and hypoxia-induced extracellular matrix remodeling. Elevated P4HA1 enhances collagen deposition and stiffening of the tumor microenvironment, promoting invasion and metastasis. Conversely, inhibition of P4HA1 suppresses collagen accumulation and fibrotic scarring. Research using P4HA1 antibody aids studies in extracellular matrix biology, collagen synthesis, and disease-associated fibrosis.
P4HA1 antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence to detect collagen-modifying enzymes.
Structurally, Prolyl 4-hydroxylase subunit alpha 1 contains a catalytic dioxygenase domain that requires Fe2+ and ascorbate as cofactors for hydroxylation. This antibody enables characterization of P4HA1's role in collagen maturation, ECM formation, and disease progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
