P53 Reporter Lentivirus

Background

TP53 encodes p53, a tumor suppressor transcription factor often described as the guardian of the genome. In response to cellular stresses such as DNA damage, oncogene activation, and hypoxia, p53 is stabilized and binds specific response elements to activate genes that drive cell cycle arrest, DNA repair, senescence, and apoptosis. Through this program, p53 prevents the propagation of damaged or potentially malignant cells. TP53 is the most frequently mutated gene in human cancer, and loss of p53 function compromises these protective responses. Because of its central role, p53 transcriptional activity is widely monitored in studies of genotoxic stress, apoptosis, and tumor suppression.

Product Description & Applications

The P53 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of p53 activity in human or mouse cells. Tandem consensus p53 response elements drive expression of a reporter (GFP, RFP, firefly luciferase, or Renilla luciferase), so that p53 activation produces a quantitative signal indicative of p53 and apoptosis pathway engagement in transduced cells. Antibiotic selection markers (puromycin or blasticidin) support establishment of stable reporter cell lines. The system is used to study genotoxic stress responses, apoptosis, and tumor suppressor signaling, and to screen modulators of p53 activity. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.

About This Product

This reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Apoptosis/p53 signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.