| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide specific to human PABPN1 was used as the immunogen for the PABPN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PABPN1 Antibody / Polyadenylate-binding protein 2 is a anti-PABPN1 Rabbit antibody Recombinant Rabbit Monoclonal clone AAHI-16 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Cytoplasm.
Key elements and design rationale
- Target: PABPN1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone AAHI-16, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P
Biological background
PABPN1 functions primarily in the nucleus, where it coordinates with other RNA-binding proteins and processing factors to promote accurate maturation of mRNA. Beyond its role in polyadenylation, PABPN1 has been implicated in alternative polyadenylation site choice, thereby influencing transcript diversity and gene regulation. Through these activities, PABPN1 contributes to global control of gene expression and adaptation to cellular conditions.
Mutations in PABPN1 cause oculopharyngeal muscular dystrophy (OPMD), a late-onset genetic disorder characterized by progressive weakening of the eyelid, pharyngeal, and proximal limb muscles. The disease is associated with expansion mutations in the polyalanine tract of PABPN1, leading to nuclear aggregation of the protein and impaired RNA processing. This connection highlights the essential role of PABPN1 in muscle cell biology and its broader significance in human health.
In addition to OPMD, altered PABPN1 function has been linked to cancer and neurodegeneration, where dysregulated RNA processing contributes to disease progression. Studies suggest that changes in PABPN1 activity may affect transcriptome profiles and cellular stress responses, making it a protein of interest in both basic and translational research. Its role in regulating mRNA maturation also positions it as a critical node in pathways that integrate transcription and translation.
The PABPN1 antibody is commonly applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to evaluate protein expression, nuclear localization, and disease-related alterations. These applications support research into RNA metabolism, muscular dystrophy, and transcriptome regulation. For scientists investigating RNA processing, muscle pathology, or gene expression control, the PABPN1 antibody provides a reliable detection reagent.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
