| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PACSIN1 recombinant protein (Position: Q76-E359) was used as the immunogen for the PACSIN1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PACSIN1 Antibody / Protein kinase C and casein kinase substrate in neurons protein 1 is a anti-PACSIN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PACSIN1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Protein kinase C and casein kinase substrate in neurons protein 1 contains an F-BAR domain that binds curved membranes and an SH3 domain that interacts with dynamin and synaptojanin. Through these interactions, PACSIN1 supports the fission of clathrin-coated vesicles during endocytosis. The PACSIN1 antibody allows detection of this neuronal scaffold protein in both cultured neurons and brain tissues, providing valuable insights into the molecular mechanisms of synaptic vesicle retrieval and recycling.
PACSIN1 is essential for maintaining proper synaptic transmission and plasticity. Its loss disrupts vesicle endocytosis and actin dynamics, leading to impaired neuronal signaling. The PACSIN1 antibody enables researchers to examine protein expression and distribution across different brain regions, supporting studies of neurotransmission and dendritic spine architecture. It also helps identify post-translational modifications, such as phosphorylation by casein kinase and protein kinase C, that regulate its membrane association and function.
In addition to synaptic processes, Protein kinase C and casein kinase substrate in neurons protein 1 has roles in endocytic trafficking in non-neuronal cells and participates in receptor internalization and signaling. Altered expression or mutation of PACSIN1 has been associated with neurological disorders including schizophrenia and neurodevelopmental delay, possibly through disrupted endocytic signaling and synaptic connectivity. The PACSIN1 antibody provides a means to analyze such pathophysiological changes at the protein level.
Experimental applications for the PACSIN1 antibody include western blotting, immunofluorescence, and immunohistochemistry, where it reveals strong neuronal and synaptic staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
