| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PAG1 was used as the immunogen for the PAG1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PAG1 Antibody / Cbp / Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 is a anti-PAG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, plasma membrane.
Key elements and design rationale
- Target: PAG1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS
Biological background
Functionally, PAG1 antibody identifies a 432-amino-acid type I transmembrane protein containing multiple tyrosine residues that undergo phosphorylation following receptor activation. Phosphorylated PAG1 binds CSK, leading to phosphorylation and inactivation of Src kinases at their C-terminal regulatory tyrosine. This mechanism provides a critical negative feedback loop that fine-tunes T cell receptor (TCR) and B cell receptor (BCR) signaling, maintaining immune homeostasis.
The PAG1 gene is located on chromosome 8q21.13 and is expressed in hematopoietic cells, neurons, and various epithelial tissues. In T lymphocytes, PAG1 is enriched in membrane microdomains and associates with LAT, LCK, and CD45, modulating activation thresholds and signaling kinetics. Beyond the immune system, PAG1 participates in neuronal development, cell adhesion, and stress response signaling pathways.
Pathologically, dysregulated PAG1 expression contributes to altered immune signaling and oncogenic kinase activation. Downregulation of PAG1 has been observed in certain lymphomas and leukemias, allowing unchecked Src kinase activity. Conversely, overexpression may suppress cell motility and proliferation. Research using PAG1 antibody supports investigations into lipid raft-associated signaling, immune synapse organization, and signal attenuation mechanisms.
PAG1 antibody is suitable for use in western blotting, immunoprecipitation, and immunofluorescence to detect native or phosphorylated PAG1 in cell lysates or tissue samples. It supports studies focused on immune receptor regulation, kinase signaling networks, and cancer biology.
Structurally, PAG1 contains an extracellular immunoglobulin-like domain, a single transmembrane segment, and a cytoplasmic tail rich in tyrosine motifs. Its lipid raft localization depends on palmitoylation and interaction with glycosphingolipids. This antibody aids in elucidating PAG1's scaffolding functions that link plasma membrane signaling to intracellular kinase regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
