| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PKC was used as the immunogen for the Pan-PKC antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Pan-PKC Antibody / PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCZ is a anti-Pan-PKC Rabbit antibody Recombinant Rabbit Monoclonal clone 32P28 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: Pan-PKC
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P28, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
The Protein kinase C family is divided into conventional, novel, and atypical classes based on their structural domains and activation requirements. Conventional isoforms such as PRKCA, PRKCB, and PRKCG require calcium and diacylglycerol for activation, while novel isoforms including PRKCD and PRKCE are calcium independent but still activated by diacylglycerol. Atypical isoforms such as PRKCZ do not respond to either calcium or diacylglycerol but are regulated by other lipid derived second messengers. Research using Pan-PKC antibody has shown that this kinase family integrates multiple extracellular signals, allowing cells to adapt rapidly to changing environments.
Aberrant Protein kinase C signaling is implicated in cancer, diabetes, cardiovascular disease, and neurological disorders. Overexpression or mutation of individual isoforms alters cell cycle progression, apoptosis thresholds, and stress responses. For example, PRKCA is frequently upregulated in breast cancer, while PRKCD contributes to apoptosis in lymphoid cells. Using Pan-PKC antibody allows researchers to detect total family expression or activation states, providing a comprehensive readout when isoform specificity is not required. This broad recognition is especially valuable in screening studies where pathway activity is assessed across multiple tissues and disease contexts.
Pan-PKC antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting enables detection of multiple isoforms in complex lysates, while immunohistochemistry highlights expression patterns across tissues such as brain, liver, and immune organs. Immunofluorescence demonstrates localization of Protein kinase C to membranes, cytoplasm, or nucleus depending on isoform activation state. Functional studies with Pan-PKC antibody reveal dynamic translocation patterns following receptor stimulation, underscoring the central role of this kinase family in signaling.
By supplying validated Pan-PKC antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
