| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PAN3 recombinant protein (Position: Q227-L887) was used as the immunogen for the PAN3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PAN3 Antibody / PAN deadenylation complex subunit 3 is a anti-PAN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PAN3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
PAN3 is encoded by the PAN3 gene located on human chromosome 13q12.13. The protein is approximately 89 kilodaltons and characterized by coiled-coil, zinc finger, and RNA-binding motifs that support its role in mRNA degradation. Within the PAN2-PAN3 complex, PAN3 acts as a scaffold protein that recruits PAN2 to poly(A)-bound PABP (poly(A)-binding protein) and stimulates deadenylase activity. This process represents the first step in mRNA decay, preceding decapping and exonucleolytic degradation.
The PAN3 antibody detects an ~96 kilodalton protein by western blot and shows cytoplasmic punctate localization consistent with processing bodies (P-bodies) and stress granules. Functional assays reveal that PAN3, together with PAN2, regulates transcript half-life during stress, differentiation, and cell cycle transitions. Depletion of PAN3 stabilizes mRNAs and alters global translation, underscoring its central role in RNA homeostasis.
Beyond mRNA decay, PAN3 contributes to early embryonic development and stress responses by coordinating the balance between translation and degradation. It interacts with the CCR4-NOT complex and RNA helicases, integrating deadenylation with other post-transcriptional mechanisms.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
