| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PANX3 recombinant protein (Position: M1-P392) was used as the immunogen for the PANX3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PANX3 Antibody / Pannexin 3 is a anti-PANX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PANX3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, FACS, ELISA
Biological background
Functionally, PANX3 antibody recognizes a 392-amino-acid integral membrane protein expressed predominantly in skin, cartilage, and bone. Pannexin 3 mediates ATP and ion fluxes that influence osteoblast differentiation, chondrocyte maturation, and keratinocyte communication. It also interacts with intracellular calcium signaling pathways, linking membrane excitability to tissue remodeling and growth.
The PANX3 gene, located on chromosome 11q24.2, encodes a protein with four transmembrane helices, two extracellular loops with conserved cysteines, and intracellular N- and C-termini. PANX3 localizes to both plasma membrane and endoplasmic reticulum, participating in calcium homeostasis and metabolic coupling. Its expression is upregulated during endochondral ossification, contributing to skeletal development.
Pathologically, abnormal PANX3 activity is implicated in osteoarthritis, cartilage degeneration, and abnormal bone formation. Overexpression enhances ATP release and promotes differentiation, while deficiency can impair mineralization and growth plate organization. In addition, pannexin signaling has been studied in cancer and inflammation for its role in autocrine ATP signaling and cell death regulation.
PANX3 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry, making it a valuable tool for studies of bone biology, differentiation, and tissue communication.
Structurally, Pannexin 3 forms hexameric channels resembling connexins but does not form gap junctions. Instead, it functions as an ATP release channel regulated by voltage, phosphorylation, and extracellular calcium. Research with this antibody helps clarify PANX3's role in skeletal physiology and pathophysiology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
