| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PARL was used as the immunogen for the PARL antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PARL Antibody / Presenilins-associated rhomboid-like protein is a anti-PARL Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PARL
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, PARL antibody identifies a 379-amino-acid protein localized to the inner mitochondrial membrane. PARL cleaves and activates key mitochondrial substrates such as PINK1 and PGAM5, initiating mitophagy and apoptosis signaling pathways. Through these actions, PARL maintains mitochondrial homeostasis and protects cells from oxidative stress. It also participates in remodeling the inner membrane during stress conditions, influencing cristae organization and mitochondrial fission-fusion balance.
The PARL gene is located on chromosome 3q27.1 and is expressed in metabolically active tissues including brain, heart, and skeletal muscle. Expression levels fluctuate with energy demand and stress, with upregulation observed during mitochondrial biogenesis and differentiation. PARL is evolutionarily conserved across eukaryotes, emphasizing its fundamental role in organelle maintenance.
Pathologically, mutations in PARL have been associated with mitochondrial dysfunction, Parkinson's disease, and metabolic disorders. Impaired PARL activity disrupts PINK1 processing, leading to defective mitophagy and accumulation of damaged mitochondria. In contrast, overexpression may alter apoptotic sensitivity by influencing cytochrome c release. Research using PARL antibody supports studies in mitochondrial biology, apoptosis regulation, and neurodegenerative disease.
PARL antibody can be validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitochondrial proteases.
Structurally, Presenilins-associated rhomboid-like protein contains seven transmembrane domains forming a proteolytic chamber within the inner mitochondrial membrane. Its catalytic serine and histidine residues form the rhomboid protease active site. The protein's C-terminal region undergoes regulated processing, modulating its activity in stress conditions. This antibody enables investigation of PARL's role in mitochondrial proteostasis and apoptosis signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
